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easygo! human il-6 one-step elisa kit  (Multi Sciences (Lianke) Biotech Co Ltd)


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    Multi Sciences (Lianke) Biotech Co Ltd easygo! human il-6 one-step elisa kit
    Easygo! Human Il 6 One Step Elisa Kit, supplied by Multi Sciences (Lianke) Biotech Co Ltd, used in various techniques. Bioz Stars score: 99/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+il/EasyGo!+Human+IL-6+One-Step+ELISA+Kit/custom%40ek106eg%4042764425
    Average 99 stars, based on 48 article reviews
    easygo! human il-6 one-step elisa kit - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: ADAR1-HNRNPL-Mediated CircCANX Decline Promotes Autophagy in Chronic Obstructive Pulmonary Disease.
    Article Snippet: .. ELISA Assay: The supernatant from the different treatment groups was collected to detect the IL-6 and IL-1β levels using a Human IL-6 High Sensitivity ELISA Kit (Multi Sciences, EK106HS) and Human IL-1β ELISA Kit (Proteintech, KE00021) according to the manufacturer’s instructions. .. The BALF of mice was used to detect the IL-6 and IL-1β levels using a Mouse IL-6 ELISA kit (Weiao Biotechnology, EM30325 M) and Mouse IL1β ELISA Kit (Multi Sciences, EK201B).

    Article Title: Nanoparticles Fueled by Enzyme for the Treatment of Hyperlipidemic Acute Pancreatitis.
    Article Snippet: .. Mesoporous silica (xfnano), 3-aminopropyl triethoxysilane (99%, APTES, Sigma-Aldrich), lipase (from porcine pancreas, Sigma-Aldrich), and glutaraldehyde (GA, 25% in water, SigmaAldrich), IL-6-specific human aptamer (sequence 5-NH2-GGT GGC AGG AGG ACT ATT TAT TTG CTT TTC T-3′) 5′ modified with the carboxyl group and 3′ modified with 6-FAM were synthesized and purified by Sangon Biotech (Shanghai, China).52,53 1-Ethyl-3-(3(dimethylamino)propyl) carbodiimide (EDC, Sigma-Aldrich), Nhydroxysuccinimide (NHS, Sigma-Aldrich), fluorescein 5(6)-isothiocyanate (FITC, Sigma-Aldrich), TAMRA-azide (FITC, SigmaAldrich), rhodamine B (Sigma-Aldrich), agarose (Sigma-Aldrich), bicinchoninic acid (BCA) protein concentration assay kit (Enhanced) (Beyotime), recombinant human IL-6 (PeproTech), FITC-labeled IL-6 (Customized by Company GenScript), human IL-6 high sensitivity ELISA Kit (MultiSciences), triglyceride content test kit (Solarbio), hemoglobin (Hb) content test kit (Solarbio), albumin content test kit (Solarbio), total bilirubin (TBIL) content test kit (Solarbio), direct bilirubin (DBIL) content test kit (Solarbio), triglyceride (Solarbio), glyceryl triacetate (≥99.0%, Sigma-Aldrich), litmus (aladdin), anti-IL-6 antibody (abcam), Poloxamer 407 (SigmaAldrich), L-arginine (Sigma-Aldrich), phosphate buffer saline (PBS), other Western blotting, and nucleic acid agarose related reagents were purchased from Beyotime. ..

    Article Title: Demethoxycurcumin mitigates inflammatory responses in lumbar disc herniation via MAPK and NF-κB pathways in vivo and in vitro.
    Article Snippet: The inflammatory radicular pain induced by lumbar disc herniation (LDH) is a serious problem worldwide.. Demethoxycurcumin (DMC) is a yellow pigment derived from turmeric.. Although it is considered a safe natural compound for managing inflammation-associated diseases, but the molecular mechanisms of LDH remain to be elucidated.

    Article Title: TNF inhibitors target a mevalonate metabolite/TRPM2/calcium signaling axis in neutrophils to dampen vasculitis in Behçet’s disease
    Article Snippet: Western Blot: TRPM2 antibody (1:500, SABbiotech, Clone: MGC133383, Cat:22689, Lot: 3519); -Actin (13E5) Rabbit mAb (1:1000, abcam, Clone: 13E5, Cat:ab8227) Immunohistochemistry: MPO (1:1000, Abcam, Clone:EPR20257, Cat: AB208670, Lot: 1041028-32) Immunofluorescence: MPO (1:500, Abcam, Clone:EPR20257, Cat: AB208670, Lot: 1041028-32); Ly-6G (1A8) Rat mAb (FITC Conjugate) (1:500, CST, Clone: 1A8, Cat: 88876S, Lot: 10); YF®594 Goat Anti-Rabbit IgG (H&L) (1:1000, UElandy, Cat: Y6107L, Lot: 210412L2). .. ELISA: human TNF- ELISA Kit (MultiScience (Lianke) Biotech, Cat: EK182-96, Lot: A18210342); human IL-6 precoated ELISA Kit (Dakewei Biotech,Cat: 1110602, Lot: 2301-2); human IFN- ELISA Kit (MultiScience (Lianke) Biotech, Cat: EK180-96, Lot: A18010543); human MPO-DNA complex ELISA Kit (MEIMIAN,Cat: MM-2467H1, Lot: 202402). ..

    Article Title: iNOS inhibitor S-methylisothiourea alleviates smoke inhalation-induced acute lung injury by suppressing inflammation and macrophage infiltration.
    Article Snippet: Objective: We investigated the effects of the inducible NO synthase (iNOS) inhibitor, S-methylisothiourea (SMT), in a mouse model of smoke inhalation-induced acute lung injury (ALI) and explored the underlying molecular

    Article Title: Effect of fermentation modification on the physicochemical characteristics and anti-aging related activities of Polygonatum kingianum polysaccharides.
    Article Snippet: In order to fully investigate the anti-aging value of the plants polysaccharides, the fermentation method was applied to modify the Polygonatum kingianum polysaccharides (PKPS), and the ultra-filtration was used to further segment the hydrolyzed polysaccharides.. It was found that the fermentation induced an increase in the in vitro anti-aging-related activities of PKPS including antioxidant, hypoglycemic and hypolipidemic activity, and cellular aging-delaying ability.. In particular, the low Mw fraction PS2-4 (10-50 kDa) separated from the fermented polysaccharide exhibited superior anti-aging activity on experimental animals.

    Article Title: Imbalance of follicular regulatory T (Tfr) cells/follicular helper T (Tfh) cells in adult patients with primary immune thrombocytopenia
    Article Snippet: .. The serum levels of IL-21 and IL-10 cytokines were detected with human IL-21/IL-10 enzyme-linked immunosorbent assay (ELISA) kit (Shanghai Lianke Biological Co., Ltd., Shanghai, China), according to the manufacturer’s instructions. ..

    Article Title: Pericentriolar material 1 promotes intestinal inflammation in ulcerative colitis by activating <scp>NLRP3</scp>/gasdermin D‐mediated macrophage pyroptosis
    Article Snippet: The concentrations of IL- 1β and IL- 18 in the supernatant of THP- 1 cells were determined by the corresponding Enzyme- linked immunosorbent assay (ELISA) kits according to the manufacturer's instructions. .. ELISA kits for human IL- 1β and human IL- 18 were acquired from Multisciences (Lianke) Biotech, Co., Ltd. (Hangzhou, China). ..

    Sequencing:

    Article Title: Nanoparticles Fueled by Enzyme for the Treatment of Hyperlipidemic Acute Pancreatitis.
    Article Snippet: .. Mesoporous silica (xfnano), 3-aminopropyl triethoxysilane (99%, APTES, Sigma-Aldrich), lipase (from porcine pancreas, Sigma-Aldrich), and glutaraldehyde (GA, 25% in water, SigmaAldrich), IL-6-specific human aptamer (sequence 5-NH2-GGT GGC AGG AGG ACT ATT TAT TTG CTT TTC T-3′) 5′ modified with the carboxyl group and 3′ modified with 6-FAM were synthesized and purified by Sangon Biotech (Shanghai, China).52,53 1-Ethyl-3-(3(dimethylamino)propyl) carbodiimide (EDC, Sigma-Aldrich), Nhydroxysuccinimide (NHS, Sigma-Aldrich), fluorescein 5(6)-isothiocyanate (FITC, Sigma-Aldrich), TAMRA-azide (FITC, SigmaAldrich), rhodamine B (Sigma-Aldrich), agarose (Sigma-Aldrich), bicinchoninic acid (BCA) protein concentration assay kit (Enhanced) (Beyotime), recombinant human IL-6 (PeproTech), FITC-labeled IL-6 (Customized by Company GenScript), human IL-6 high sensitivity ELISA Kit (MultiSciences), triglyceride content test kit (Solarbio), hemoglobin (Hb) content test kit (Solarbio), albumin content test kit (Solarbio), total bilirubin (TBIL) content test kit (Solarbio), direct bilirubin (DBIL) content test kit (Solarbio), triglyceride (Solarbio), glyceryl triacetate (≥99.0%, Sigma-Aldrich), litmus (aladdin), anti-IL-6 antibody (abcam), Poloxamer 407 (SigmaAldrich), L-arginine (Sigma-Aldrich), phosphate buffer saline (PBS), other Western blotting, and nucleic acid agarose related reagents were purchased from Beyotime. ..

    Modification:

    Article Title: Nanoparticles Fueled by Enzyme for the Treatment of Hyperlipidemic Acute Pancreatitis.
    Article Snippet: .. Mesoporous silica (xfnano), 3-aminopropyl triethoxysilane (99%, APTES, Sigma-Aldrich), lipase (from porcine pancreas, Sigma-Aldrich), and glutaraldehyde (GA, 25% in water, SigmaAldrich), IL-6-specific human aptamer (sequence 5-NH2-GGT GGC AGG AGG ACT ATT TAT TTG CTT TTC T-3′) 5′ modified with the carboxyl group and 3′ modified with 6-FAM were synthesized and purified by Sangon Biotech (Shanghai, China).52,53 1-Ethyl-3-(3(dimethylamino)propyl) carbodiimide (EDC, Sigma-Aldrich), Nhydroxysuccinimide (NHS, Sigma-Aldrich), fluorescein 5(6)-isothiocyanate (FITC, Sigma-Aldrich), TAMRA-azide (FITC, SigmaAldrich), rhodamine B (Sigma-Aldrich), agarose (Sigma-Aldrich), bicinchoninic acid (BCA) protein concentration assay kit (Enhanced) (Beyotime), recombinant human IL-6 (PeproTech), FITC-labeled IL-6 (Customized by Company GenScript), human IL-6 high sensitivity ELISA Kit (MultiSciences), triglyceride content test kit (Solarbio), hemoglobin (Hb) content test kit (Solarbio), albumin content test kit (Solarbio), total bilirubin (TBIL) content test kit (Solarbio), direct bilirubin (DBIL) content test kit (Solarbio), triglyceride (Solarbio), glyceryl triacetate (≥99.0%, Sigma-Aldrich), litmus (aladdin), anti-IL-6 antibody (abcam), Poloxamer 407 (SigmaAldrich), L-arginine (Sigma-Aldrich), phosphate buffer saline (PBS), other Western blotting, and nucleic acid agarose related reagents were purchased from Beyotime. ..

    Synthesized:

    Article Title: Nanoparticles Fueled by Enzyme for the Treatment of Hyperlipidemic Acute Pancreatitis.
    Article Snippet: .. Mesoporous silica (xfnano), 3-aminopropyl triethoxysilane (99%, APTES, Sigma-Aldrich), lipase (from porcine pancreas, Sigma-Aldrich), and glutaraldehyde (GA, 25% in water, SigmaAldrich), IL-6-specific human aptamer (sequence 5-NH2-GGT GGC AGG AGG ACT ATT TAT TTG CTT TTC T-3′) 5′ modified with the carboxyl group and 3′ modified with 6-FAM were synthesized and purified by Sangon Biotech (Shanghai, China).52,53 1-Ethyl-3-(3(dimethylamino)propyl) carbodiimide (EDC, Sigma-Aldrich), Nhydroxysuccinimide (NHS, Sigma-Aldrich), fluorescein 5(6)-isothiocyanate (FITC, Sigma-Aldrich), TAMRA-azide (FITC, SigmaAldrich), rhodamine B (Sigma-Aldrich), agarose (Sigma-Aldrich), bicinchoninic acid (BCA) protein concentration assay kit (Enhanced) (Beyotime), recombinant human IL-6 (PeproTech), FITC-labeled IL-6 (Customized by Company GenScript), human IL-6 high sensitivity ELISA Kit (MultiSciences), triglyceride content test kit (Solarbio), hemoglobin (Hb) content test kit (Solarbio), albumin content test kit (Solarbio), total bilirubin (TBIL) content test kit (Solarbio), direct bilirubin (DBIL) content test kit (Solarbio), triglyceride (Solarbio), glyceryl triacetate (≥99.0%, Sigma-Aldrich), litmus (aladdin), anti-IL-6 antibody (abcam), Poloxamer 407 (SigmaAldrich), L-arginine (Sigma-Aldrich), phosphate buffer saline (PBS), other Western blotting, and nucleic acid agarose related reagents were purchased from Beyotime. ..

    Purification:

    Article Title: Nanoparticles Fueled by Enzyme for the Treatment of Hyperlipidemic Acute Pancreatitis.
    Article Snippet: .. Mesoporous silica (xfnano), 3-aminopropyl triethoxysilane (99%, APTES, Sigma-Aldrich), lipase (from porcine pancreas, Sigma-Aldrich), and glutaraldehyde (GA, 25% in water, SigmaAldrich), IL-6-specific human aptamer (sequence 5-NH2-GGT GGC AGG AGG ACT ATT TAT TTG CTT TTC T-3′) 5′ modified with the carboxyl group and 3′ modified with 6-FAM were synthesized and purified by Sangon Biotech (Shanghai, China).52,53 1-Ethyl-3-(3(dimethylamino)propyl) carbodiimide (EDC, Sigma-Aldrich), Nhydroxysuccinimide (NHS, Sigma-Aldrich), fluorescein 5(6)-isothiocyanate (FITC, Sigma-Aldrich), TAMRA-azide (FITC, SigmaAldrich), rhodamine B (Sigma-Aldrich), agarose (Sigma-Aldrich), bicinchoninic acid (BCA) protein concentration assay kit (Enhanced) (Beyotime), recombinant human IL-6 (PeproTech), FITC-labeled IL-6 (Customized by Company GenScript), human IL-6 high sensitivity ELISA Kit (MultiSciences), triglyceride content test kit (Solarbio), hemoglobin (Hb) content test kit (Solarbio), albumin content test kit (Solarbio), total bilirubin (TBIL) content test kit (Solarbio), direct bilirubin (DBIL) content test kit (Solarbio), triglyceride (Solarbio), glyceryl triacetate (≥99.0%, Sigma-Aldrich), litmus (aladdin), anti-IL-6 antibody (abcam), Poloxamer 407 (SigmaAldrich), L-arginine (Sigma-Aldrich), phosphate buffer saline (PBS), other Western blotting, and nucleic acid agarose related reagents were purchased from Beyotime. ..

    Protein Concentration:

    Article Title: Nanoparticles Fueled by Enzyme for the Treatment of Hyperlipidemic Acute Pancreatitis.
    Article Snippet: .. Mesoporous silica (xfnano), 3-aminopropyl triethoxysilane (99%, APTES, Sigma-Aldrich), lipase (from porcine pancreas, Sigma-Aldrich), and glutaraldehyde (GA, 25% in water, SigmaAldrich), IL-6-specific human aptamer (sequence 5-NH2-GGT GGC AGG AGG ACT ATT TAT TTG CTT TTC T-3′) 5′ modified with the carboxyl group and 3′ modified with 6-FAM were synthesized and purified by Sangon Biotech (Shanghai, China).52,53 1-Ethyl-3-(3(dimethylamino)propyl) carbodiimide (EDC, Sigma-Aldrich), Nhydroxysuccinimide (NHS, Sigma-Aldrich), fluorescein 5(6)-isothiocyanate (FITC, Sigma-Aldrich), TAMRA-azide (FITC, SigmaAldrich), rhodamine B (Sigma-Aldrich), agarose (Sigma-Aldrich), bicinchoninic acid (BCA) protein concentration assay kit (Enhanced) (Beyotime), recombinant human IL-6 (PeproTech), FITC-labeled IL-6 (Customized by Company GenScript), human IL-6 high sensitivity ELISA Kit (MultiSciences), triglyceride content test kit (Solarbio), hemoglobin (Hb) content test kit (Solarbio), albumin content test kit (Solarbio), total bilirubin (TBIL) content test kit (Solarbio), direct bilirubin (DBIL) content test kit (Solarbio), triglyceride (Solarbio), glyceryl triacetate (≥99.0%, Sigma-Aldrich), litmus (aladdin), anti-IL-6 antibody (abcam), Poloxamer 407 (SigmaAldrich), L-arginine (Sigma-Aldrich), phosphate buffer saline (PBS), other Western blotting, and nucleic acid agarose related reagents were purchased from Beyotime. ..

    Recombinant:

    Article Title: Nanoparticles Fueled by Enzyme for the Treatment of Hyperlipidemic Acute Pancreatitis.
    Article Snippet: .. Mesoporous silica (xfnano), 3-aminopropyl triethoxysilane (99%, APTES, Sigma-Aldrich), lipase (from porcine pancreas, Sigma-Aldrich), and glutaraldehyde (GA, 25% in water, SigmaAldrich), IL-6-specific human aptamer (sequence 5-NH2-GGT GGC AGG AGG ACT ATT TAT TTG CTT TTC T-3′) 5′ modified with the carboxyl group and 3′ modified with 6-FAM were synthesized and purified by Sangon Biotech (Shanghai, China).52,53 1-Ethyl-3-(3(dimethylamino)propyl) carbodiimide (EDC, Sigma-Aldrich), Nhydroxysuccinimide (NHS, Sigma-Aldrich), fluorescein 5(6)-isothiocyanate (FITC, Sigma-Aldrich), TAMRA-azide (FITC, SigmaAldrich), rhodamine B (Sigma-Aldrich), agarose (Sigma-Aldrich), bicinchoninic acid (BCA) protein concentration assay kit (Enhanced) (Beyotime), recombinant human IL-6 (PeproTech), FITC-labeled IL-6 (Customized by Company GenScript), human IL-6 high sensitivity ELISA Kit (MultiSciences), triglyceride content test kit (Solarbio), hemoglobin (Hb) content test kit (Solarbio), albumin content test kit (Solarbio), total bilirubin (TBIL) content test kit (Solarbio), direct bilirubin (DBIL) content test kit (Solarbio), triglyceride (Solarbio), glyceryl triacetate (≥99.0%, Sigma-Aldrich), litmus (aladdin), anti-IL-6 antibody (abcam), Poloxamer 407 (SigmaAldrich), L-arginine (Sigma-Aldrich), phosphate buffer saline (PBS), other Western blotting, and nucleic acid agarose related reagents were purchased from Beyotime. ..

    Saline:

    Article Title: Nanoparticles Fueled by Enzyme for the Treatment of Hyperlipidemic Acute Pancreatitis.
    Article Snippet: .. Mesoporous silica (xfnano), 3-aminopropyl triethoxysilane (99%, APTES, Sigma-Aldrich), lipase (from porcine pancreas, Sigma-Aldrich), and glutaraldehyde (GA, 25% in water, SigmaAldrich), IL-6-specific human aptamer (sequence 5-NH2-GGT GGC AGG AGG ACT ATT TAT TTG CTT TTC T-3′) 5′ modified with the carboxyl group and 3′ modified with 6-FAM were synthesized and purified by Sangon Biotech (Shanghai, China).52,53 1-Ethyl-3-(3(dimethylamino)propyl) carbodiimide (EDC, Sigma-Aldrich), Nhydroxysuccinimide (NHS, Sigma-Aldrich), fluorescein 5(6)-isothiocyanate (FITC, Sigma-Aldrich), TAMRA-azide (FITC, SigmaAldrich), rhodamine B (Sigma-Aldrich), agarose (Sigma-Aldrich), bicinchoninic acid (BCA) protein concentration assay kit (Enhanced) (Beyotime), recombinant human IL-6 (PeproTech), FITC-labeled IL-6 (Customized by Company GenScript), human IL-6 high sensitivity ELISA Kit (MultiSciences), triglyceride content test kit (Solarbio), hemoglobin (Hb) content test kit (Solarbio), albumin content test kit (Solarbio), total bilirubin (TBIL) content test kit (Solarbio), direct bilirubin (DBIL) content test kit (Solarbio), triglyceride (Solarbio), glyceryl triacetate (≥99.0%, Sigma-Aldrich), litmus (aladdin), anti-IL-6 antibody (abcam), Poloxamer 407 (SigmaAldrich), L-arginine (Sigma-Aldrich), phosphate buffer saline (PBS), other Western blotting, and nucleic acid agarose related reagents were purchased from Beyotime. ..

    Western Blot:

    Article Title: Nanoparticles Fueled by Enzyme for the Treatment of Hyperlipidemic Acute Pancreatitis.
    Article Snippet: .. Mesoporous silica (xfnano), 3-aminopropyl triethoxysilane (99%, APTES, Sigma-Aldrich), lipase (from porcine pancreas, Sigma-Aldrich), and glutaraldehyde (GA, 25% in water, SigmaAldrich), IL-6-specific human aptamer (sequence 5-NH2-GGT GGC AGG AGG ACT ATT TAT TTG CTT TTC T-3′) 5′ modified with the carboxyl group and 3′ modified with 6-FAM were synthesized and purified by Sangon Biotech (Shanghai, China).52,53 1-Ethyl-3-(3(dimethylamino)propyl) carbodiimide (EDC, Sigma-Aldrich), Nhydroxysuccinimide (NHS, Sigma-Aldrich), fluorescein 5(6)-isothiocyanate (FITC, Sigma-Aldrich), TAMRA-azide (FITC, SigmaAldrich), rhodamine B (Sigma-Aldrich), agarose (Sigma-Aldrich), bicinchoninic acid (BCA) protein concentration assay kit (Enhanced) (Beyotime), recombinant human IL-6 (PeproTech), FITC-labeled IL-6 (Customized by Company GenScript), human IL-6 high sensitivity ELISA Kit (MultiSciences), triglyceride content test kit (Solarbio), hemoglobin (Hb) content test kit (Solarbio), albumin content test kit (Solarbio), total bilirubin (TBIL) content test kit (Solarbio), direct bilirubin (DBIL) content test kit (Solarbio), triglyceride (Solarbio), glyceryl triacetate (≥99.0%, Sigma-Aldrich), litmus (aladdin), anti-IL-6 antibody (abcam), Poloxamer 407 (SigmaAldrich), L-arginine (Sigma-Aldrich), phosphate buffer saline (PBS), other Western blotting, and nucleic acid agarose related reagents were purchased from Beyotime. ..

    Cell Culture:

    Article Title: Demethoxycurcumin mitigates inflammatory responses in lumbar disc herniation via MAPK and NF-κB pathways in vivo and in vitro.
    Article Snippet: The inflammatory radicular pain induced by lumbar disc herniation (LDH) is a serious problem worldwide.. Demethoxycurcumin (DMC) is a yellow pigment derived from turmeric.. Although it is considered a safe natural compound for managing inflammation-associated diseases, but the molecular mechanisms of LDH remain to be elucidated.



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    Multi Sciences (Lianke) Biotech Co Ltd human il-6 elisa kit
    Concentration-dependent change in the humoral inflammatory response following incubation with Escherichia coli ( E. coli ) in the ex vivo whole blood model. a Absolute plasma concentrations of IL-6, <t>IL-8,</t> and MMP9 determined by enzyme-linked immunosorbent assay. b Normalized values and EC 50 curve fit by BuC=0% and 50 000 CFU/ml E. coli= 100%, respectively, for IL-6, IL-8, and MMP9 as indicated by EC 50 (%) on the respective Y-axis. BuC indicates buffer control after 60 min incubation; numbers on the X-axis indicate E. coli bacteria in concentrations of 2000 to 50 000 CFU/ml after 60 min incubation; LPS indicates lipopolysaccharide (LPS) 100 ng/ml after 60 min incubation. Values are shown as median and interquartile range. n =8. Statistical analysis was performed using the Kruskal-Wallis test with Dunn’s post-hoc test, comparing all shown concentrations of E. coli bacteria and 100 ng/ml LPS with BuC. P -values are indicated above the respective data points. ⁎ P <0.05, ⁎⁎ P <0.01, ⁎⁎⁎ P <0.001. CFU. Colony-forming units; IL. Interleukin; MMP9. Matrix metallopeptidase 9.
    Human Il 6 Elisa Kit, supplied by Multi Sciences (Lianke) Biotech Co Ltd, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Multi Sciences (Lianke) Biotech Co Ltd human il-8 elisa kit
    Concentration-dependent change in the humoral inflammatory response following incubation with Escherichia coli ( E. coli ) in the ex vivo whole blood model. a Absolute plasma concentrations of IL-6, <t>IL-8,</t> and MMP9 determined by enzyme-linked immunosorbent assay. b Normalized values and EC 50 curve fit by BuC=0% and 50 000 CFU/ml E. coli= 100%, respectively, for IL-6, IL-8, and MMP9 as indicated by EC 50 (%) on the respective Y-axis. BuC indicates buffer control after 60 min incubation; numbers on the X-axis indicate E. coli bacteria in concentrations of 2000 to 50 000 CFU/ml after 60 min incubation; LPS indicates lipopolysaccharide (LPS) 100 ng/ml after 60 min incubation. Values are shown as median and interquartile range. n =8. Statistical analysis was performed using the Kruskal-Wallis test with Dunn’s post-hoc test, comparing all shown concentrations of E. coli bacteria and 100 ng/ml LPS with BuC. P -values are indicated above the respective data points. ⁎ P <0.05, ⁎⁎ P <0.01, ⁎⁎⁎ P <0.001. CFU. Colony-forming units; IL. Interleukin; MMP9. Matrix metallopeptidase 9.
    Human Il 8 Elisa Kit, supplied by Multi Sciences (Lianke) Biotech Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Multi Sciences (Lianke) Biotech Co Ltd human il-2 elisa kit
    Concentration-dependent change in the humoral inflammatory response following incubation with Escherichia coli ( E. coli ) in the ex vivo whole blood model. a Absolute plasma concentrations of IL-6, <t>IL-8,</t> and MMP9 determined by enzyme-linked immunosorbent assay. b Normalized values and EC 50 curve fit by BuC=0% and 50 000 CFU/ml E. coli= 100%, respectively, for IL-6, IL-8, and MMP9 as indicated by EC 50 (%) on the respective Y-axis. BuC indicates buffer control after 60 min incubation; numbers on the X-axis indicate E. coli bacteria in concentrations of 2000 to 50 000 CFU/ml after 60 min incubation; LPS indicates lipopolysaccharide (LPS) 100 ng/ml after 60 min incubation. Values are shown as median and interquartile range. n =8. Statistical analysis was performed using the Kruskal-Wallis test with Dunn’s post-hoc test, comparing all shown concentrations of E. coli bacteria and 100 ng/ml LPS with BuC. P -values are indicated above the respective data points. ⁎ P <0.05, ⁎⁎ P <0.01, ⁎⁎⁎ P <0.001. CFU. Colony-forming units; IL. Interleukin; MMP9. Matrix metallopeptidase 9.
    Human Il 2 Elisa Kit, supplied by Multi Sciences (Lianke) Biotech Co Ltd, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Concentration-dependent change in the humoral inflammatory response following incubation with Escherichia coli ( E. coli ) in the ex vivo whole blood model. a Absolute plasma concentrations of IL-6, IL-8, and MMP9 determined by enzyme-linked immunosorbent assay. b Normalized values and EC 50 curve fit by BuC=0% and 50 000 CFU/ml E. coli= 100%, respectively, for IL-6, IL-8, and MMP9 as indicated by EC 50 (%) on the respective Y-axis. BuC indicates buffer control after 60 min incubation; numbers on the X-axis indicate E. coli bacteria in concentrations of 2000 to 50 000 CFU/ml after 60 min incubation; LPS indicates lipopolysaccharide (LPS) 100 ng/ml after 60 min incubation. Values are shown as median and interquartile range. n =8. Statistical analysis was performed using the Kruskal-Wallis test with Dunn’s post-hoc test, comparing all shown concentrations of E. coli bacteria and 100 ng/ml LPS with BuC. P -values are indicated above the respective data points. ⁎ P <0.05, ⁎⁎ P <0.01, ⁎⁎⁎ P <0.001. CFU. Colony-forming units; IL. Interleukin; MMP9. Matrix metallopeptidase 9.

    Journal: Military Medical Research

    Article Title: The cellular response capacity (CRC) as a novel immunomonitoring approach in sepsis

    doi: 10.1016/j.mmr.2026.100010

    Figure Lengend Snippet: Concentration-dependent change in the humoral inflammatory response following incubation with Escherichia coli ( E. coli ) in the ex vivo whole blood model. a Absolute plasma concentrations of IL-6, IL-8, and MMP9 determined by enzyme-linked immunosorbent assay. b Normalized values and EC 50 curve fit by BuC=0% and 50 000 CFU/ml E. coli= 100%, respectively, for IL-6, IL-8, and MMP9 as indicated by EC 50 (%) on the respective Y-axis. BuC indicates buffer control after 60 min incubation; numbers on the X-axis indicate E. coli bacteria in concentrations of 2000 to 50 000 CFU/ml after 60 min incubation; LPS indicates lipopolysaccharide (LPS) 100 ng/ml after 60 min incubation. Values are shown as median and interquartile range. n =8. Statistical analysis was performed using the Kruskal-Wallis test with Dunn’s post-hoc test, comparing all shown concentrations of E. coli bacteria and 100 ng/ml LPS with BuC. P -values are indicated above the respective data points. ⁎ P <0.05, ⁎⁎ P <0.01, ⁎⁎⁎ P <0.001. CFU. Colony-forming units; IL. Interleukin; MMP9. Matrix metallopeptidase 9.

    Article Snippet: For the samples of the ex vivo whole blood model, the plasma concentrations of matrix metallopeptidase 9 (MMP9, #DY911, R&D Systems, Minneapolis, USA), IL-6 (#555220, BD Biosciences, San Jose, USA), and IL-8 (#DY208, R&D Systems) were measured in citrate-anticoagulated plasma using enzyme-linked immunosorbent assay according to the respective manufacturer’s instructions.

    Techniques: Concentration Assay, Incubation, Ex Vivo, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Control, Bacteria

    Diagnostic performance for the detection of bacteremia, analyzing the neutrophil phenotype by determining the median fluorescence intensity (MFI) and the cellular response capacity (CRC) in comparison with traditional markers of humoral inflammation (IL-6, IL-8, MMP9). a Comparison of receiver operating characteristic (ROC) at 10,000 CFU/ml Escherichia coli ( E. coli ) with the respective 95% confidence interval (CI) and P -value, and half-maximal effective concentration (EC 50 ) as a function of the E. coli concentration. b Detailed comparison of the EC 50 as a function of the E. coli concentration. c Exemplary comparison of EC 50 curve fit after normalization as indicated by EC 50 (%) on the respective Y-axis to BuC=100% and 50 000 CFU/ml E. coli =0% for the humoral marker IL-6 (the IL-6 values were multiplied by −1 before EC 50 calculation to facilitate comparability with the CRC) and the change in neutrophil phenotype represented by CD11b CRC. BuC indicates buffer control after 60 min incubation; numbers on the X-axis of c indicate E. coli bacteria in concentrations of 2000 to 50 000 CFU/ml after 60 min incubation. Values are shown as median and interquartile range. n =8. Statistical analysis was performed using the Kruskal-Wallis test with Dunn’s post-hoc test, evaluating the EC 50 of IL-8, MMP9, the MFI, and CRC of CD10, CD11b, and CD62L in comparison to the EC 50 of IL-6. P -values are indicated above the respective data points. ⁎ P <0.05. CFU. Colony-forming units; IL. Interleukin; MMP9. Matrix metallopeptidase 9.

    Journal: Military Medical Research

    Article Title: The cellular response capacity (CRC) as a novel immunomonitoring approach in sepsis

    doi: 10.1016/j.mmr.2026.100010

    Figure Lengend Snippet: Diagnostic performance for the detection of bacteremia, analyzing the neutrophil phenotype by determining the median fluorescence intensity (MFI) and the cellular response capacity (CRC) in comparison with traditional markers of humoral inflammation (IL-6, IL-8, MMP9). a Comparison of receiver operating characteristic (ROC) at 10,000 CFU/ml Escherichia coli ( E. coli ) with the respective 95% confidence interval (CI) and P -value, and half-maximal effective concentration (EC 50 ) as a function of the E. coli concentration. b Detailed comparison of the EC 50 as a function of the E. coli concentration. c Exemplary comparison of EC 50 curve fit after normalization as indicated by EC 50 (%) on the respective Y-axis to BuC=100% and 50 000 CFU/ml E. coli =0% for the humoral marker IL-6 (the IL-6 values were multiplied by −1 before EC 50 calculation to facilitate comparability with the CRC) and the change in neutrophil phenotype represented by CD11b CRC. BuC indicates buffer control after 60 min incubation; numbers on the X-axis of c indicate E. coli bacteria in concentrations of 2000 to 50 000 CFU/ml after 60 min incubation. Values are shown as median and interquartile range. n =8. Statistical analysis was performed using the Kruskal-Wallis test with Dunn’s post-hoc test, evaluating the EC 50 of IL-8, MMP9, the MFI, and CRC of CD10, CD11b, and CD62L in comparison to the EC 50 of IL-6. P -values are indicated above the respective data points. ⁎ P <0.05. CFU. Colony-forming units; IL. Interleukin; MMP9. Matrix metallopeptidase 9.

    Article Snippet: For the samples of the ex vivo whole blood model, the plasma concentrations of matrix metallopeptidase 9 (MMP9, #DY911, R&D Systems, Minneapolis, USA), IL-6 (#555220, BD Biosciences, San Jose, USA), and IL-8 (#DY208, R&D Systems) were measured in citrate-anticoagulated plasma using enzyme-linked immunosorbent assay according to the respective manufacturer’s instructions.

    Techniques: Diagnostic Assay, Fluorescence, Comparison, Concentration Assay, Marker, Control, Incubation, Bacteria

    Clinical specifications and parameters over all time points of the sepsis cohort. a Suspected infection cause of sepsis. b Distribution of the individual score points of the Sequential Organ Failure Assessment (SOFA) score. c Total SOFA score. d-h Traditional and humoral markers of inflammation: leukocytes and neutrophil-lymphocyte ratio ( d ), C-reactive protein (CRP) and procalcitonin (PCT) ( e ), interleukin-6 (IL-6) and interleukin-8 (IL-8) ( f ), serum amyloid A (SAA) and calprotectin ( g ), matrix metallopeptidase 9 (MMP9) and myeloperoxidase (MPO) ( h ). Values are shown as median and interquartile range. n =14. CNS. Central nervous system; HV. Healthy volunteers.

    Journal: Military Medical Research

    Article Title: The cellular response capacity (CRC) as a novel immunomonitoring approach in sepsis

    doi: 10.1016/j.mmr.2026.100010

    Figure Lengend Snippet: Clinical specifications and parameters over all time points of the sepsis cohort. a Suspected infection cause of sepsis. b Distribution of the individual score points of the Sequential Organ Failure Assessment (SOFA) score. c Total SOFA score. d-h Traditional and humoral markers of inflammation: leukocytes and neutrophil-lymphocyte ratio ( d ), C-reactive protein (CRP) and procalcitonin (PCT) ( e ), interleukin-6 (IL-6) and interleukin-8 (IL-8) ( f ), serum amyloid A (SAA) and calprotectin ( g ), matrix metallopeptidase 9 (MMP9) and myeloperoxidase (MPO) ( h ). Values are shown as median and interquartile range. n =14. CNS. Central nervous system; HV. Healthy volunteers.

    Article Snippet: For the samples of the ex vivo whole blood model, the plasma concentrations of matrix metallopeptidase 9 (MMP9, #DY911, R&D Systems, Minneapolis, USA), IL-6 (#555220, BD Biosciences, San Jose, USA), and IL-8 (#DY208, R&D Systems) were measured in citrate-anticoagulated plasma using enzyme-linked immunosorbent assay according to the respective manufacturer’s instructions.

    Techniques: Infection